Monday, November 14, 2005

New gun against TBC (Armas contra la Tuberculosis).


Lombardi (1992:4-56), documented conglomerates of visible acid alcohol resistant bacilli by microscope of light, originating in samples of lung of a mummy of Nasca-Peru. 1000 years after Christ (a.C). Later, Salo et al (1994: 2091-94), identified segments of DNA, specific of Mycobacterium tuberculosis, in prepared of lung abcesss and lymphatics nodules, of an adult mummified woman from the southeast area of Peru (lower part of the valley of Osmore, near the coast of Ilo. 1040 years a.C). 97 peers of identical bases to those of present M. tuberculosis, confirmed the final presence of Tuberculosis (TB), in peruvian pre-hispanic times. Since then the TB does not cease to afflict the Peruvians and all the remainder world. 8 million new cases and 2 million deaths by TB, occur each year.

Until it does little the middle of cultivation employed been used to was the Lowenstein Jensen to cultivate : M. tuberculosis. This method delayed 40 days in enabling growth of colonies. But, suddenly happened in Lima-Peru, that a team conformed by David Moore and Jonathan Friedland of the Imperial one College of London, RHG Gillman of the John Hopkins University-USA- and L. Caviedes, Emicco Gonzales and Hugo Garcia of the Peruvian University Cayetano Heredia, working together in urban areas of Lima, they have managed to create a new one method to detect M. tuberculosis, whose main characteristics are: 1) sputum is employed 2) causes the fast growths of M. Tuberculosis: a liquid medium, instead of solid (L.Jensen), where after 40 days you obtained colonies to be visualized 3) you can see the M.tuberculosis bacilli, on an average of 7-11days, using microscope of inverted light 4)it is possible to add drugs to the liquid medium, to measure sensitivity to them 5) their total cost is 2,5 dolares 6) it does need a lot of experience.

Nuevas armas contra la TBC

Lombardi (1992:4-56), documentó conglomerados de bacilos acido alcohol resistentes visibles al microscopio de luz, procedentes de muestras de pulmón de una momia de Nasca-Peru. 1000 años después de Cristo (d.C). Mas tarde Salo et al (1994, 2091-94), identificaron segmentos de DNA, especificos de Mycobacterium tuberculosis, en preparados de lesiones pulmonares y nodulos linfaticos, de una mujer adulta momificada procedente del area suroriental del Peru (parte baja del valle de Osmore, cerca de la costa de Ilo.1040 años d.C). 97 pares de bases idénticas a los de M tuberculosis actuales, confirmaron la presencia definitiva de Tuberculosis (TB), en epocas prehispánicas peruanas. Desde entonces la TB no cesa de afligir a los peruanos y al resto del mundo. 8 millones de casos nuevos y 2 millones de muertes por TB, ocurren cada año.

Hasta hace poco se empleaba el medio de cultivo solido de Lowenstein Jensen para cultivar al M. tuberculosis. Este metodo tardaba 40 dias en posibilitar el crecimiento de colonias. Pero he aquí de pronto que en Lima-Peru, un equipo conformado por David Moore y Jonathan Friedland del Imperial College de Londres, RHG Gillman del John Hopkins University-USA- y L. Caviedes, Emicco Gonzales y Hugo Garcia de la Universidad Peruana Cayetano Heredia de Peru, trabajando juntos en areas urbanas de Lima, han logrado crear un nuevo metodo para detectar M tuberculosis, cuyas caracteristicas son: 1) se emplea esputo 2) se hace crecer al M. tuberculosis en medio liquido(donde crece mas rapido), en lugar del solido (L.Jensen) donde tras 40 dias, se visualizaban colonias 3) entre los 7-11 dias, se aprecian enmarañados de bacilos M.tuberculosis, usando microscopio de luz invertida 4) es posible añadir drogas al medio liquido para medir susceptibilidad a ellas 5) su costo total es 2,5 dolares 6) no se necesita mucha experiencia.
Refrencias:

1-Lombardi Almonacin Guido,1992.Detección de M. tuberculosis en una momia de la cultura Nasca con mal de Pott. Tesis de Bachiller en Medicina.Universidad Peruana Cayetano Heredia, Lima
2-Salo,L., Aufderheide,C.A., Buikstra, J. and Helcomb, A.T. 1994. Identification of M. Tuberculosis DNA in a pre-Columbian Peruvian mummy. PNAS. 91:2091-94.
3-Caviedes, L,
et al.2000. Rapid efficient detection and drug susceptibility testing of M.tuberculosis in sputum by microscopic observation of broth cultures. J. Clin Microbiology. 38 (3):1203-8.

2 comments:

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