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Wednesday, April 30, 2008

ARTIFICIAL SUPERSILK



Andreas Bausch, S. Rammensee (Technical University/Munich) and others, know that spiders stored silk proteins in aqueous solutions contained in inner organs (spinneret, spinning ducts, just of a few micrometers), turning them into insoluble fibres in seconds, thanks to irreversible aggregation of silk proteins, formed by crystalline regions, separated by amorphous unions of high stretchability, matching the properties of carbon fibre and polyaramid. They also know that silk fibers are potentially stronger than steel (bulletproof vests, lightweight parachutes, strong ropes), superelastic, biodegradable (fishing nets, thread for sutures, cover for wounds facilitating healing process).

The german team created liquid microconducts, where silk proteins: eADF3 and eADF4, developed through recombinant genetic engineering (engineered araneus diadematus fibroin) were converted into fibers, taking advantage of prior knowledge acquired by Nexia Biotechnologies which obtained silk fibers of 10 -60 um generated by cells of goats, carrying certain genes from spiders (although the natural spider silk fiber measure: 2.5-4 μm). The unfolded artificial fibers created by the german team were dissolved in liquid solutions, squeezed through holes increasingly smaller, emerging folded and insoluble, in seconds. Changes in pH and ionic conditions conditioned aggregation of the 2 proteins. The assembly of fibers eADF3 was induced in the presence of an elongation flow. Surprisingly eADF4 fibers were formed only in combination with eADF3. Thanks to this work, the germans proposed a model with early steps and aggregation of silk proteins in microscopic systems, although without detailing the physical or mechanical properties or explaining complex changes during the process of spinning.

SUPERSEDA ARTIFICIAL

Andreas Bausch, R. Ramensee (Technical University/Munich) y otros, saben que las arañas almacenan proteínas de sus sedas en solucciones acuosas contenidas en órganos internos (spinning ducts, de apenas unos pocos micrómetros), convirtiéndolas en segundos en fibras insolubles, merced a la agregación irreversible de proteinas de la seda, constituidas por regiones cristalinas, separadas por uniones amorfas de gran capacidad de estiramiento, igualando las propiedades de la fibra de carbono y el polyaramid. Saben también que las fibras son potencialmente más fuertes que el acero (chalecos antibalas, paracaídas livianos, sogas superfuertes), superelásticas, biodegradables (redes de pesca, hilos para suturas, cubiertas de heridas facilitadoras de la cicatrización).

Los alemanes crearon microconductos liquidos, en el que las proteinas de la seda : eADFi3 y eADF4, elaboradas mediante ingenieria genética recombinante (engineered Araneus diadematus fibroin), fueron convertidas en fibras, aprovechando conocimientos previos adquiridos por Nexia Biotechnologies que creó fibras de seda de 10-60 μm generadas por células de cabras, portadoras de ciertos genes de arañas (aunque los de la araña miden : 2.5-4 μm). Las fibras artificiales creadas por los alemanes (no dobladas), disueltas en solucciones liquidas fueron exprimidas en agujeros cada vez más y mas pequeños emergiendo dobladas e insolubles en segundos. Cambios en las condiciones ionicas y el pH condicionaron la agregación de las 2 proteinas. El ensamblaje de las fibras eADF3 fue inducida en presencia de un flujo de elongacion. Sorprendentemente las fibras eADF4 se formaron solo en combinacion con eADF3. Merced a este trabajo, los alemanes proponen un modelo de formación de pasos tempranos y agregación de proteinas de la seda en regímenes microscópicos, aunque sin detallar las propiedades físicas o mecánicas ni explicar los complejos cambios ocurridos durante el proceso de hilado.

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